Analytical validation of the PAM50-based Prosigna Breast Cancer Prognostic Gene Signature Assay and nCounter Analysis System using formalin-fixed paraffin-embedded breast tumor specimens

Table 2 Variance components for the five pooled RNA samples across 108 replicates

Pooled RNA sample	Mean ROR score	Variance component (%)					Total variance	Total SD
Pooled RNA sample	Mean ROR score	Reagent lot	Site	Operator	Run	Within-run	Total variance	Total SD
Basal-like	55.4	0.059 (20%)	0.000 (0%)	0.000 (0%)	0.046 (15%)	0.194 (65%)	0.299 (100%)	0.55
HER2-enriched	76.2	0.165 (37%)	0.000 (0%)	0.000 (0%)	0.000 (0%)	0.277 (63%)	0.442 (100%)	0.66
Luminal A	31.4	0.010 (2%)	0.000 (0%)	0.000 (0%)	0.134 (30%)	0.296 (67%)	0.44 (100%)	0.66
Luminal B 1	55.0	0.105 (18%)	0.000 (0%)	0.000 (0%)	0.046 (8%)	0.426 (74%)	0.576 (100%)	0.76
Luminal B 2	64.8	0.119 (21%)	0.014 (2%)	0.000 (0%)	0.064 (11%)	0.380 (66%)	0.576 (100%)	0.76

ISSN: 1471-2407