Figure 5

Both NKX3.1 protein and wild type NKX3.1 binding sites are required for the transcriptional repressor function of TMPRSS2 promoter upstream sequences. (A) Schematic representation of NBS1 and NBS4 sequences marking predicted NKX3.1 binding elements in brackets. Core recognition sequences with transversion mutations are underlined in the wild type (wt) and in the mutant (mt) sequences. (B) Relative luciferase units (RLU) of wild type and mutant NKX3.1 binding sites were assayed in reporter constructs in the presence (+) of heterologously expressed NKX3.1 or in the presence of control vector (-). Asterisk symbols mark significant (P < 0.05) reductions in reporter gene expression.