Figure 2
MK-2206 affects cell proliferation and cell death in vitro. A) MTT analysis shows reduction in cell proliferation on treatment with MK-2206. B) DNA fragmentation showing an increase in cell death with increasing concentration of MK-2206. C) Western blot analysis of various apoptotic members as pBad (Ser136), XIAP and Survivin. D) IP for 14-3-3 to determine the interaction with pBad (Ser136) and Bad showing a loss in the interaction on treatment with MK-2206. E) IP for Bad to determine the interaction with anti-apoptotic protein Bcl-xL. (* = P < 0.01 and ** = P < 0.001).