Figure 6
Phospho-aspirin-2 induces p53 acetylation and Dmp1 expression. A: Upper: PA-2 induced the acetylation of p53 in MDA-MB-231 (K373, K379 and K382) and BT-20 (K373 and K379) cells. Lower: PA-2 induced p53 acetylation in MDA-MB-231 (K382) and BT-20 (K373) xenografts. B: Left panel: SIRT1 overexpression prevents p53 acetylation (K382) by PA-2. Right panel: SIRT1 overexpression attenuated PA-2-induced apoptosis in MDA-MB-231 cells. Western blot confirmed the overexpression of SIRT1. C: Upper: PA-2 1.5 x IC50 increased the expression of Dmp1 in MDA-MB-231 and BT-20 cells. Lower: PA-2 increased Dmp1 expression in xenografts, as indicated by western blot (left) and immunohistochemistry (right). Two representative tissue sections are shown. *, p < 0.02, compared to control; magnification 200X. Immunoblots were performed with β-actin as the loading control.