Figure 3
Maltonis effect on cell cycle and apoptosis. (A) Cell cycle evaluation of RD/18, U-2OS and TC-71 cells after 48 hours of treatment with maltonis 3 or 10 μM. Data are expressed as mean percentages of two independent experiments. Percentage of cells in the G1 or G2/M phase are significantly different after treatment compared to the respective controls (P < 0.01E-16 by Chi Square test for 10 μM). (B) Annexin V-PI analysis, expressed as percentage of live (black), apoptotic (dark grey) or necrotic (light grey) cells, in a panel of sarcoma after 72 hours treatment with 3 or 10 μM maltonis. Values are representative of 3 independent experiments. Percentage of apoptotic or necrotic cells are significantly different after treatment compared to the respective controls (P ≤ 0.02 by Chi Square).