Figure 6From: Integrity of the LXXLL motif in Stat6 is required for the inhibition of breast cancer cell growth and enhancement of differentiation in the context of progesterone Stat6 is required for the inhibition of G1/S cell cycle progression by progesterone. Synchronized T47D cells, transfected with control or Stat6 siRNAs (500Â ng) and treated with or without progesterone, were harvested after 12Â h or 24Â h to determine cell cycle phase distribution (A) or Western blotting using either an antibody raised against pRB phosphorylated at Ser807/811 or an antibody recognizing specifically the underphosphorylated form of pRB (B). Representative Western blotting results are shown in B. pRB-Ser807-811, pRB phosphorylated on the Ser807/811 residue; pRB, hypophosphorylated pRB. The MDA-MB-468 cell line, deficient in pRB expression, was used as a negative control of pRB staining.Back to article page