Figure 2From: OCT4 increases BIRC5 and CCND1 expression and promotes cancer progression in hepatocellular carcinoma BIRC5 promoter activity in HCC cells. (A) Scheme of binding sites for transcription factors in the BIRC5 proximal promoter region. (B) Cell lines, including the indicated parental, adenovirus-infected and shRNA-transfected cells, were seeded on 24-well plates at a density of 5 × 104 cells/well and transfected with the BIRC5 promoter-driven luciferase plasmid pSRVN-Luc (200 ng/well). The relative activity of BIRC5 promoter in HCC cells was measured and shown in histograms; *, P < 0.05; **, P < 0.01. (C) Cells were seeded on 96-well plates at a density of 5 × 103 cells/well, transfected with the BIRC5 promoter-driven EGFP plasmid pSRVN-EGFP (2 μg/well), and observed under a fluorescent microscope 48 h later, original magnification 200 × .Back to article page