Figure 4
From: IL-22 is related to development of human colon cancer by activation of STAT3
IL-22 promotes tumor growth and metastasis in vivo. TILs were isolated from two CC patients and co-transplanted subcutaneously into nude mice with Hct-116 cells. Tumor growth and metastasis was investigated. A: Different IL-22 expression levels of TILs1 and TILs2 analyzed by FCMs. B1: Tumor tissues obtained from nude mice from each group. B2: Comparison of tumor volume of each group (unpaired t-test). C1: IL-22 positive cell distribution in tumor tissues of each group investigated by IHC. C2: Average integrated optical density (IOD) was obtained by analyzing five fields for each slide evaluated by Image-Pro Plus software (version 5.0) for IHC staining of IL-22. D: Western-blot detection of expression of p-STAT3 (S727), total STAT3, Bcl-xl, CyclinD1, and VEGF, all normalized to β-actin for every 3 samples in each group. E: IHC staining of CEA in lymph nodes around the tumor beneath the skin of nude mice. E1 (×40), E2, E3 (×100) and E4 (×200), ** represented P < 0.01.