Figure 4

p53-induced expression of mature and pre-miR genes. RT-qPCR were performed in HCT116 p53^+/+(black bars), HCT116 p53^−/− (white bars) and MCF7 (gray bars) cells upon doxorubicin (A, B) or Nutlin (C) for 24 hours. The expression of the processed mature miR (A) or of the pre-miR RNA (B, C) was tested. p21 mRNA expression was measured as p53-dependent positive control. PVT1 non-coding RNA expression levels were measured as additional evidence of p53-dependent expression of miR-1204 and miR-1206. Results are presented as fold of induction with respect to the mock condition. Bars plot average and standard deviations of three independent experiments. (D) Western Blot establishing stabilization of p53 protein in doxorubicin and Nutlin treated cells and the induction of the p53 target gene p21. GAPDH was used [6] as reference.