Figure 7

Western blot analysis of the expression of β-catenin and Ror2 in PANC-1 and BXPC-3 cells with indicated treatments. (a) Treatment of pancreatic cancer cells with recombinant Wnt5a (200 ng) resulted in an increase in the nuclear β-catenin level. Lamin A/C was used as a nuclear internal control. N: nuclear fraction; C: cytoplasmic fraction. (b) The Ror2 protein level of pancreatic cancer cells remained unchanged in the presence of recombinant Wnt5a with or without CIAP. GAPDH was used as an internal control. CIAP, calf intestinal alkaline phosphatase. (c) PANC-1 cells were transfected with β-catenin or control siRNA 24 h before exposure to recombinant Wnt5a. After incubation for another 24 h, Western blot analysis was performed to examine the protein expression of indicated EMT markers. The bar graph shows the results of densitometric analysis. (d) Transwell invasion assay was performed to test the invasiveness of PANC-1 cells transfected with β-catenin or control siRNA 24 h before treatment with recombinant Wnt5a. The bar graph represents the data from three independent experiments.