Figure 6

Impact of the combined treatment on NF-κB activity and p53 mediated apoptosis. NF-κB reporter activities in HT-1080 cells, 6 h and 10 h post-treatment as indicated. The firefly reporter activities were normalized to Renilla luciferase (A). Real-time RT-PCR analysis of p53 (B), p21 (C) and PUMA (D) expression in HT-1080 cells following treatment for 8 h and 10 h. Actin was used for normalization. Western blot analysis of p53 serine 15 phosphorylation 6 h after treatment (E). Lamin served as loading control for the nuclear, and tubulin for the cytoplasmic fraction. Time course of caspase 9 activity 14 h, 18 h, 26 h after treatment as indicated (F). (* p < 0.05, ** p < 0.01, *** p < 0.001).