From: Three dimensional multiphoton imaging of fresh and whole mount developing mouse mammary glands
Method | Laser | Emission | Imaging parameters | Image |
---|---|---|---|---|
Excitation | ||||
Live Tissue | 860 nm | 390-465 nm | Ch2 BP 390–465 IR | Figure 2 |
(5.8%) | 500-550 nm | Ch3 BP 500–550 IR | ||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Live Tissue | 860 nm | 390-465 nm | ChS BP 393-436 | Figure 3A-C |
(5.8%) | Â | ChD | ||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
 | 860 nm | 390-465 nm | ChS BP 393-436 | Figure 3D |
(5.8%) | 500-550 nm | Ch2 BP 500-550 | ||
ChD | ||||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Live Tissue | 860 nm | 390-465 nm | Ch2 BP 390–465 IR | Figure 4 (Live Tissue) |
(5.8%) | 500-550 nm | Ch3 BP 500–550 IR | ||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm | 390-465 nm | Ch2 BP 390–465 IR | Figure 4 (Whole Mount) |
(5.8%) | 500-550 nm | Ch3 BP 500–550 IR | ||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 543 nm (50.5%) | 565-615 nm | Ch3 BP 565–615 IR | Figure 5A,B |
ChD | ||||
MBS: HFT 488/543/633 | ||||
DBS1: NFT 635 VIS | ||||
DBS2: NFT 545 | ||||
FW1: None | ||||
Carmine Alum WM | 750 nm (2.1%) | 565-615 nm | Ch3 BP 565–615 IR | Figure 5C |
MBS: HFT KP650 | ||||
DBS1: NFT KP545 | ||||
DBS2: NFT 545 | ||||
FW1: None | ||||
BF NDD | ||||
Carmine Alum WM | 750 nm (5.8%) | 565-615 nm | Ch3: BP 565–615 IR | Figure 6 |
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 545 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Figure 7 |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 393-436 | ChS BP 393-436 | Figure 7B |
ChD | ChD (unfiltered) | SHG-B/SHG-F image only | ||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Figure 8 |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Figure 9 |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 750, 800, 830, 860, 890, 950 nm (0.1%) | Lambda scan | ChS:361-704 | Figure 10 |
361-704 nm | MBS: HFT KP650 | |||
DBS1: None | ||||
DBS2: None | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Figure 11 (Ex 860/ Em 650–710) |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
 | 750 nm (5.8%) | 565-615 nm | Ch3: BP 565–615 IR | Figure 11 (Ex 750/ Em 565–615) |
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 545 | ||||
FW1: None | ||||
Unstained WM | 735, 860, 960 nm (0.1%) | Lambda scan | ChS:361-704 | Figure 12A, B |
361-704 nm | MBS: HFT KP650 | |||
DBS1: None | ||||
DBS2: None | ||||
FW1: None | ||||
 | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Figure 12C-E |
500-530 nm | Ch3 BP 500–530 IR | |||
or | or | |||
650-710 nm ChD | Ch3 BP 650–710 IR ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
 | DBS1: Mirror | |||
DBS2: NFT 490 | ||||
FW1: None | ||||
Unstained WM | 860, 890 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 13: Figure S11 |
500-530 nm | Ch3 BP 500–530 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
 | 800, 890 nm (0.1%) | Lambda scan | ChS:361-704 | Additional file 13: Figure S11 |
361-704 nm | MBS: HFT KP650 | |||
DBS1: None | ||||
DBS2: None | ||||
FW1: None | ||||
Unstained WM | 800, 890 nm (0.1%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 13: Figure S11 |
500-530 nm | Ch3 BP 500–530 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 1: Figure S1 |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Live Tissue | 860 nm (0.1%) | Lambda scan | ChS:361-704 | Additional file 2: Figure S2 |
361-704 nm | MBS: HFT KP650 | |||
DBS1: None | ||||
DBS2: None | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 4: Figure S3 |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860, nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 5: Figure S4 |
650-710 nm | Ch3 BP 650–710 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm | Lambda scan | ChS:361-704 | Additional file 7: Figure S5A,B |
(0.1%) | 361-704 nm | MBS: HFT KP650 | Additional file 11: Figure S9B | |
DBS1: None | ||||
DBS2: None | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 7: Figure S5C |
650-710 nm | Ch3 BP 650–710 IR | |||
or | or | |||
565-615 | Ch3 BP 565–615 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 8: Figure S6 |
650-710 nm | Ch3 BP 650–710 IR | |||
or | or | |||
565-615 | Ch3 BP 565–615 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Carmine Alum WM | 860 nm (5.8%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 9: Figure S7 |
650-710 nm | Ch3 BP 650–710 IR | |||
or | or | |||
565-615 | Ch3 BP 565–615 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Unstained WM | 860 nm | Lambda scan | ChS:361-704 | Additional file 10: Figure S8 |
(0.1%) | 361-704 nm | MBS: HFT KP650 | ||
DBS1: None | ||||
DBS2: None | ||||
FW1: None | ||||
Unstained WM | 860 nm (0.1%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 11: Figure S9A, C |
500-530 nm | Ch3 BP 500–530 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None | ||||
Unstained WM | 860 nm (0.1%) | 390-465 nm | Ch2 BP 390–465 IR | Additional file 12: Figure S10 |
500-530 nm | Ch3 BP 500–530 IR | |||
ChD | ChD (unfiltered) | |||
MBS: HFT KP650 | ||||
DBS1: Mirror | ||||
DBS2: NFT 490 | ||||
FW1: None |