Figure 6

Detection of abnormal TEB lumen. A Carmine Alum stained TEB from a HAI-1 mouse was sampled using Z-slice spacing of 1 μm for 74 μm. A. A 3D reconstruction was made using Zeiss AIM software. The arrow points to a defect on the surface of the TEB which was stained with Carmine Alum. Imaging was performed using Ex 750 nm and Em bandpass 565–615 nm. B. An individual Z-slice at z = 24 μm and orthogonal XZ and YZ slices illustrate the central lumen of the duct (asterisk) and the abnormal pocket of cells forming an extra lumen near the tip of the TEB (arrow). C. A conventional bright field image from an HAI mouse taken using a Nikon E600 upright microscope equipped with a 10X/ 0.3 N.A. Nikon lens. Scale bars = 100 μm.