Figure 6

Additive effect of survivin ssiRNA and doxorubicin on the treatment of lung metastases. (a) Dose dependent effect of doxorubicin (1, 2.5 or 10 mg/kg) on lung metastasis growth assessed by the overall mean lung weight relative to the body weight of the mice. n = 9 animals per group. (b) Body weight measurement from the beginning to the end of the treatment was evaluated to determine the toxicity of doxorubicin treatments. (c) Schematic representation of the treatment procedure with doxorubicin alone, survivin ssiRNA alone, or both. (d) Overall mean lung weight relative to the body weight of the mice after an alternated treatment of doxorubicin and survivin ssiRNA with PEI, compared to treatment with doxorubicin or survivin ssiRNA alone. n = 9 animals per group. P values were calculated; * p<0.05; **p<0.01.