Figure 1

Inhibition of survivin and cyclin B1 expression in B16-F10 cells. (a, b) mRNA expression level of cyclin B1 and survivin analyzed by branched DNA 16 h after transfection with ssiRNAs and PEI. Cyclin B1 and survivin levels are expressed relative to GAPDH. (c) Protein level of cyclin B1 and survivin analyzed by Western blot 24, 48 and 72 h after transfection with a negative control ssiRNA (2) or ssiRNAs against cyclin B1 or survivin (3) (50 nM, N/P=8). Non transfected cells (1) were also loaded. GAPDH was used as a loading control. (d) In vitro 5’-RACE analysis of RNA extracted from B16-F10 cells either untransfected (1) or 16 h after transfection with negative control (2), cyclin B1 or survivin (3) ssiRNAs and PEI (50 nM, N/P 8). Top part, schematic representation of the 5’-RACE procedure.