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Table 2 Percentages of lymphocyte populations and their subset composition in pleural fluid assessed in healthy subjects and in patients. Results given as median (interquartile range)

From: Defect in recruiting effector memory CD8+T-cells in malignant pleural effusions compared to normal pleural fluid

 

METS

BPLAE

MPM

Healthy subjects

Lymphocyte populations a

  Total CD3+ T-cells

80.5 (76–87.5)

85.2 (81–90.1)

77 (69–84)

85 (80–88)

  CD4+ T-cells

63.2 (53.8-72)

63.5 (52.5-72)

54.2 (44–62.5)

30.0 (21–32)

  CD8+ T-cells

19 (15–25)

22 (16.4-29)

25 (19.2-33.5)

53 (45–59)

  CD4+/CD8+ ratio

3.8 (2.6-4.8)

3.2 (2.2-4.5)

2.2 (1.5-2.9)

0.59 (0.48-0.67)

  Treg (CD4low+CD25bright+)b

4.4 (3–5.9)

4.4 (2.4-5.3)

3.1

2.2 (1.6-3.7)

  NK cells (CD3─CD56+)

3.5 (2.2-7.5)

2.8 (2–4)

10 (3.4-12.5)

16.0 (12.4-24.1)

  → % of CD16 + NK cells

30 (20–59)

18 (1–35)

26 (14–54)

6% (3–8)

CD4 + T cell subsets b

  Naïve

22.7 (14.7-29)

25.7 (14.3-37)

11.2 (3.8-27)

1.6 (1–2.4)

  TCM

47.4 (30–62)

53.5 (42.9-60)

40.5 (27.5-51)

13.1 (9.8-19.6)

  TEM

21 (11.4-38.2)

18.6 (10.2-22)

19.2 (9.3-32)

85.1 (77.2-88.9)

  TTD

0.6 (0.3-1.4)

1.3 (0.5-1.7)

0.8 (0.3-1.6)

0.7 (0.3-1)

CD8 + T cell subsets b

  Naïve

15.2 (7.1-37.3)

26 (22–43.4)

11.2 (3.9-29.6)

1.4 (0.9-2.1)

  TCM

22.1 (12–34.7)

27.4 (17.8-44)

11.6 (7.2-28.3)

2.3 (1.6-4.7)

  TEM

19.7 (4.3-45.3)

16.1 (10.7-36)

18.6 (5.6-49.2)

84.7 (83.1-87)

  TTD

12.7 (5.6-22.3)

13.7 (8–22.9)

7.4 (2.4-20.7)

9.5 (8.5-12.2)

  1. aAs a percentage of the total lymphocytes (defined as CD45bright, low side scatter).
  2. bAs a percentage of the total CD4+ or CD8+ T-cell population. The total may add up to slightly less or more than 100% because medians of individual subsets are given. the interquartile range for the MPM group could not be safely calculated due to the low number of cases; T CM central-memory cells, T EM effector memory cells, T TD terminally-differentiated effector memory.