Figure 4

Effects of rapamycin on growth of lymphatic endothelial cells. A, Growth-inhibitory effects of various concentrations of rapamycin (1-1000 ng/ml) on SV-LEC and HMEC-1A cells. Optical density results are presented as means ± SD of 3 independent experiments. (* - p < 0.05 vs. control;** - p < 0.01 vs. control; one-way ANOVA with Tukey's multiple comparison post-hoc test). B, Western blot analysis showing an inhibition of the mTOR signaling pathway by rapamycin (100 ng/ml, 72h) in SV-LEC and HMEC-1A cells. C – Control; R – Rapamycin (100 ng/ml, 72h).