Figure 4
The role of Sox2 and Twist1 in RU cells. (A) MCF7-RU cells were subjected to either scramble siRNA, Sox2 siRNA, Twist1 siRNA treatment, or both before cell invasion assay. siRNA knockdown of Sox2 in MCF7-RU cells significantly increased the invasiveness, whereas siRNA knockdown of Twist1 resulted in a significant decrease in invasiveness; Simultaneous knockdown of Sox2 and Twist1 largely abrogated the suppressive effect of Sox2 on invasiveness. Triplicate experiments were performed. A representative experiment is shown (mean ± standard deviation) (n = 3). One-way ANOVA was used to calculated statistics. (B) By western blot analysis, Sox2 siRNA and Twist1 siRNA treatment dramatically decreased the expression level of Sox2 and Twist1, respectively. (C) Cell viability was measured by the MTS assay. No significant change was observed between the negative control and various treatments. (D) By quantitative RT-PCR, the expression level of E-cadherin was measured. Cells treated with double scramble siRNA were used as a negative control and data is presented as percentages of control. Statistical significance was determined by one-way ANOVA.