Figure 4
Cell growth in RPS4X-depleted OVCAR-3 and SK-OV-3 cells. A, cell growth of OVCAR-3 cells transfected with siCtrl, siRPS4X-A and siRPS4X-D molecules. Cells were transfected with the indicated siRNA sequences. The next day 10,000 cells were seeded in 60-mm plates and appropriate plates were counted every other day using a hemacytometer. B, Cell growth of SK-OV-3 cells transfected with siCtrl, siRPS4X-A and siRPS4X-D molecules. Cells were transfected with the indicated siRNA sequences. The next day 50,000 cells were seeded in 60-mm plates and appropriate plates counted every other day using a hemacytometer. C, Histogram representing the growth rate of OVCAR-3 transfected cells (from at least three transfections for each siRNA sequence) calculated from the growth curves in A. Error bars represent the standard deviation. (Unpaired student t-test: P = 7.6 × 10-5 for siRPS4X-A vs siCTRL and P =2.4 × 10-6 for siRPS4X-D vs siCTRL). D, Histogram representing the growth rate of SK-OV-3 transfected cells (from at least three transfections for each siRNA sequences) calculated from the growth curves in B. Error bars represent the standard deviation (Unpaired student t-test: P = 3.9 × 10-7 for siRPS4X-A vs siCTRL and P = 8.7 × 10-6 for siRPS4X-D vs siCTRL). Growth rates were estimated as described in materials and methods. E, Percentage of OVCAR-3 and SK-OV-3 transfected cells in each phase of the cell cycle. Cells were transfected with the indicated constructs in duplicates and subjected to FACS analysis 72 h later. (The siRPS4X represent data from cells transfected with siRPS4X-A and siRPS4X-D performed in duplicata). Data are the mean ± SE.