Figure 4

The effect of Mito-ChM on intracellular ATP levels in MCF-7, MDA-MB-231 and MCF-10A cells. (A) The MCF-7, MDA-MB-231 and MCF-10A cells seeded in 96-well plates were treated with Mito-ChM (1–20 μM) as indicated for 1–8 h. After treatment, cells were washed with complete media and either assayed immediately, or returned to cell culture incubator for (B) 24 h, (C) 48 h, or (D) 72 h. Intracellular ATP levels were measured using a luciferase-based assay. Data are represented as a percentage of control (non-treated) cells after normalization to total cellular protein for each well. The calculated absolute values of ATP (nmol ATP/mg protein) for MCF-7, MDA-MB-231 and MCF-10A cells are shown in Additional file 3: Tables S2, S3 and S4, respectively.