Figure 4

Combinatorial effects of sEphB4-HSA and Bevacizumab on tumor vasculature and tumor cells. 211H tumors were treated as in Figure 3 for one week. Tumors were then harvested for immunofluorescence analysis. Tumor vessel density was evaluated by CD31 staining. Pericyte recruitment to tumor vessel was evaluated by colocalization of NG2-positive pericytes and CD31-positive endothelial cells. Cell proliferative index was evaluated by Ki67 staining and apoptosis was assessed with TUNEL staining. At least 4 images from each analysis were used for quantification and statistical analysis. *, P<0.05; **, P<0.01; ***, P<0.002. Error bars indicate standard error of mean.