Figure 4

Effect of equol and 4-OHT on pro-apoptotic protein (A) and caspase (B) expression. MCF-7 were treated for 48 h with equol (100 μM) and 4-OHT (10 μM) with and without Z-VAD-FMK (20 μM) (A), or without Z-VAD-FMK (B), and whole cell extracts were prepared. Protein expression was then analyzed by western blot. Data are representative of three individual experiments. C, vehicle control; E, Equol (100 μM); T, 4-OHT (10 μM), E + T, Equol (100 μM) + 4-OHT (10 μM).