Figure 5

ODAM inhibits AKT activation in MDA-MB-231 breast cancer cells but not in BT-549 breast cancer cells that lack PTEN expression. A) Western blot analysis of AKT activation in lysates of control and ODAM-expressing MDA-MB-231 cells. Whole cell lysates were probed with the indicated antibodies as given in the methods. B) Growth assay of control and ODAM-expressing BT-549 breast cancer cells. Values represent the mean relative cell mass ± 1 S.D. from four replicate wells after 6 days in culture. C) Western blot analysis of AKT phosphorylation/activation in whole cell lysates of control and ODAM-expressing BT-549 cells. D) Matrigel adhesion assay of control and ODAM-expressing BT-549 cells (**, p< 0.01). Values represent the mean O.D. ± 1 S.D. for six replicates.