Figure 4

Hyaluronan synthase (HAS) 1–3 expression in benign (a-c) and dysplastic (d-f) nevi, in situ melanomas (g-i), superficial (j-l) and deep melanomas (m-o) and lymph node (LN) metastases (p-r). Black arrows in (g-h) and (j-k) point to tumor cells in in situ melanomas (g-h) and superficial melanomas (j-k) stained positively for HAS1 (g, j) and HAS2 (h, k). White dash lines in (g-i) delineate the border between the tumor cells and the stroma in in situ melanomas. Red arrow in (q) and in (r) point to stroma of LN metastases negative for HAS2 and HAS3, respectively. Scale bar in (a) 100 μm.