Figure 1

NURR1 is weakly expressed in cancerous breast epithelium, but strongly expressed in the normal breast epithelium. A) Immunohistochemical staining of breast tissue microarrays reveals expression of NURR1 in tissue cores correlating to normal and malignant breast. Images typical of scoring are depicted, with normal breast tissue shown in the lower panel. B) Each tissue core was scored independently and the mean for normal and cancerous epithelium compared using Mann-Whitney U-Test, p = 0.0016. Hematoxylin and eosin staining of each core is available at http://www.biomax.us/tissue-arrays/Breast/BR953 C) NURR1 expression was determined by Western immunoblot of protein lysates derived from normal breast epithelium, cancerous breast epithelium, and established breast cancer cell lines (MDA-MB-468, MDA-MB-231).