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Figure 6 | BMC Cancer

Figure 6

From: Effects of Δ40p53, an isoform of p53 lacking the N-terminus, on transactivation capacity of the tumor suppressor protein p53

Figure 6

Δ40p53 protects TAp53 from Hdm2-mediated degradation in Saos-2 cells. TAp53 plasmid was co-transfected with Δ40p53 and Hdm2 in Saos-2 cells. The level of expression of the proteins was analyzed by immunoblotting using DO-7 and PAb1801 antibodies for the detection of TAp53 and Δ40p53. The co-transfection of TAp53 together with Hdm2 results in the degradation of TAp53. Levels of Δ40p53 are not decreased by co-transfection with Hdm2. Co-transfection of the three factors results in the maintenance of detectable levels of TAp53 with increased phosphorylation on Serine 15 even in the presence of Hdm2.

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BMC Cancer

ISSN: 1471-2407

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