Figure 1
From: Antitumor activities of ATP-competitive inhibitors of mTOR in colon cancer cells
Effects of rapamycin, NVP-BEZ235 and PP242 on mTORC1 and mTORC2 activity in colon cancer cells. LS174T, DLD-1 and SW480 colon cancer cells were treated with increasing concentrations of rapamycin, NVP-BEZ235 or PP242 for six hours. Cells were lysed and analyzed by Western blot for phospho-S6 ribosomal protein and phospho-Akt as a read-out for mTORC1 and mTORC2 activity respectively. Total S6 ribosomal protein and total Akt were used as loading controls. The illustrated blots are representative of three independent experiments.