Figure 5

Serum starvation induces proliferation arrest in normal SDM104 cells and protects them from CDDP cytotoxicity in vitro. Flow cytometry analysis of SDM104 cells of untreated (A) or serum-starved for 24 hours (B). C, the quantification of flow cytometry results of SDM104 cells of the untreated control, or treated with CDDP alone, serum starvation alone, or both together is shown (n=3). D, MTT assays (n=6) were performed to examine the viability of SDM104 cells of the untreated control and those treated by CDDP alone, serum starvation alone (S), or both together in vitro (** P<0.002). E, the quantification of the results of anti-pATM-Ser1981 immuno-staining in SDM104 cells of untreated control, treated by CDDP alone, serum starvation alone, or both together is shown (** P<0.002, n=3). F, Western blot results with antibodies against pATM-Ser1981 (pATM), p53-Ser15 (p53-S15), p53 and p21 in protein extracts from the untreated control, or treated with CDDP alone, serum starvation alone, or both together are shown. G, Western blot results with antibodies against pAMPK, AMPK, p53, p21, pACC and ACC in protein extracts from untreated control, treated with Compound C (C.C) alone, serum starvation alone, or both together. β-Actin and vinculin were used as loading controls.