Figure 4

Immunohistochemical findings of the pyloric gland adenoma and adjacent bile duct lesions. The pyloric gland adenoma (A, HE) immunohistochemically expressed focal MUC1 (B), but no MUC2 (C). MUC5AC (D) was positive predominantly in the superficial luminal cell layers, whereas MUC6 (E) was expressed throughout. The tumour cell also expressed vascular endothelial growth factor receptor (VEGF) (F), but no CD10 (G) and CDX2 (H). Nuclear p53 (I) was positive, p16 (J) was focally observed, and p21 (K) was positive. Ki67 (L) was observed in approximately 25% (x100).