Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape

Table 1 Multiplex qMSP results using different TaqMan buffer systems ^*

TaqMan Buffer System
Primers	Parameter	EpiTect MethyLight Mix (Qiagen)	iQ Multiplex Powermix (Bio-Rad)^**	Genotyping Master Mix (ABI)	QuantiTect Multiplex Mix (Qiagen)
ACTB	Cq	27.19±0.093	26.712±0.072	28.448±0.933	27.972±0.032
	ΔRn	0.244±0.004	2.655±0.003	0.791±0.090	0.170±0.003
CADM1	Cq	28.216±0.130	27.485±0.038	28.463±0.341	28.888±0.103
	ΔRn	0.267±0.006	2.722±0.038	1.160±0.056	0.182±0.004
MAL	Cq	26.432±0.170	25.593±0.030	29.499±0.837	26.759±0.053
	ΔRn	0.371±0.022	2.662±0.026	1.003±0.203	0.282±0.023
Hsa-miR-124-2	Cq	27.062±0.164	25.861±0.144	29.330±0.826	27.545±0.021
	ΔRn	0.630±0.010	6.505±0.029	0.691±0.125	0.514±0.014

^* Triplicate analyses of 50 ng of bisulfite treated SiHa DNA. Mean values are indicated ± standard deviations.
^** Threshold 0.1.

ISSN: 1471-2407