Figure 2

Effects of Buserelin on actin polmerization in MDA cells. A. Buserelin (10^-7M)-stimulated relative F-actin levels (in arbitrary units) in adherent MDA cells transfected with the empty vector, the WT GnRHR or the GnRHR-DesK191 cDNAs, as determined by fluorometry. Cells expressing the WT and DesK191 GnRHRs exhibited a significant increase in polymerized actin in response to the GnRHa. In each group (cells transfected with the empty vector, WT GnRHR or GnRHR-DesK191), basal F-actin (i.e. no treatment) was set to 1.0 and Buserelin-stimulated levels were expressed relative to this (horizontal line). B. Relative F-actin levels measured by flow cytometry in suspended MDA cells transfected with the empty vector, the WT GnRHR or the GnRHR-DesK191 cDNA constructs. A significant decrease in polymerized actin was observed in response to the GnRHa. C. Representative images from confocal microscopy of Buserelin (10^-7M)-stimulated MDA cells transfected with the empty vector, the WT GnRHR or the GnRHR-DesK191 cDNAs. Compared with untreated cells (lower panel) an increase in stress fibers (arrows) was apparent in WT GnRHR and GnRHR-DesK191 cells exposed to the GnRHa (upper panel). Bar: 20μm. The results shown in A and B are the means ± SEM from 3 independent experiments. ** p< 0.05; *** p<0.05.