Figure 1

Schematic construction of oncolytic HSV1-GFP and HSV1-hGM-CSF. The two oncolytic HSV1 vectors were developed from 17+ strain. First, the ICP47 gene was removed from the virus genome by pdICP47-eGFP and pdICP47. Then, the GFP expression cassette from pdICP34.5-eGFP was inserted into both sites for ICP34.5 genes to generate HSV1-GFP. The hGM-CSF expression cassette from pdICP34.5-hGM-CSF was used to replace GFP expression cassettes in HSV1-GFP to create HSV1-hGM-CSF.