Figure 5

PTPRZ1 regulates tumor progression in a SCLC xenograft mouse model. A and B, in vivo growth of SCLC NCI-H69 expressing shLUC (blue diamonds) and shZ1#2 (red squares) in SCID mice. The loss of PTPRZ1 in cells expressing shZ1#2 inhibited tumor growth as compared to cells expressing the control shLUC. Tumor size (mm³) over period (A) and gross tumor pathology 5 weeks post-transplant are shown (B). Scale bar indicates 10 mm. C and D, Deficient in vivo growth of tumors was confirmed using H69 cells expressing shZ1#3 (C) and H1930 cells expressing shZ1#2. Control SCLC cells with shLUC are shown as blue diamonds while cells with shZ1 are shown as red squares. Error bars are SD. Asterisk, P < 0.05; double asterisk, P < 0.01; both using Student’s t test.