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Figure 3 | BMC Cancer

Figure 3

From: Junction region of EWS-FLI1 fusion protein has a dominant negative effect in Ewing’s Sarcoma in vitro

Figure 3

Bacterial expression and purification of recombinant full length EWS-FLI1 . A: Coomassie stained SDS PAGE gel picture showing,expression of Full Length Trx -EWS-FLI1-His tag Protein in insoluble fraction.Lane 1 represents protein ladder, Lane2 represents soluble fraction , Lane 3 represents insoluble fraction. B: Confirmation of EWS-FLI1 protein expression by immunoblotting with anti FLI1 antibody. Lane 1: soluble fraction, Lane 2:insoluble fraction. C: Coomassie stained SDS PAGE gel picture of Ni-NTA Purification of full length EWS-FLI1 protein in denaturing condition.Lane1: Protein ladder Lane 2: Uninduced cell lysate, Lane 3: Induced cell lysate , Lane 4: Flow Through, Lane 5: Wash and Lane 6: Elute.

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BMC Cancer

ISSN: 1471-2407

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