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Table 4 Verification of overall variability and variability of the EP scores, C q values, and normalized ΔC q values at the molecular-pathological laboratory at the Charité

From: Decentral gene expression analysis: analytical validation of the Endopredict genomic multianalyte breast cancer prognosis test

 

Standard deviations

 

Cq

ΔCq

EP

variables

   

 day

0.034

0.032

0.093

 day time

0.062

0.073

0.074

 sample position

<0.001

0.016

<0.001

 reagent lot

0.182

0.031

<0.001

 operator

0.035

0.073

0.041

total variable noise

0.198

0.114

0.125

replicate noise

0.131

0.085

0.126

total noise

0.238

0.142

0.178

  1. Different operating conditions (variable noise, replicate noise and total noise) are indicated as standard deviations of the Cq values, ΔCq values, and the EP scores.
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BMC Cancer

ISSN: 1471-2407

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