Figure 3
Effect of CypA on tumorigenesis. A, CypA depletion inhibited the proliferation of 95C and A549 cells. OD490 values were determined daily at predetermined time points. The data are expressed as means ± SEM of triplicate values from three separate experiments. *P<0.05, **P<0.01 (t-test). 95C, 95C-MOCK, 95C-KD cell doubling-time was 24h, 26h, 31h respectively. And cell doubling-time of A549, A549-MOCK, A549-KD was 43h, 44h, 49h respectively. B, the effect of 239836 on cell proliferation was detected by MTS assay. The data are expressed as means ± SEM. **P<0.01 (t-test) C, CypA-assisted 95C and A549 cells avoided density-dependent growth inhibition. Colony formation after 10 days was quantified by counting the number of colonies per well. The values represent the mean number of colonies±SEM from three separate wells. **P<0.01 (t-test). D, The knockdown of CypA suppressed the anchorage-independent proliferation of 95C and A549 cells. Colony formation after 21 days was quantified by counting the number of colonies per well. The values represent the mean number of colonies (the mean±SEM) from three separate wells. **P<0.01 (t-test).