Figure 3

Effects of genotoxic agents on the cell cycle. 48 hours after 90 min treatment with genotoxic drugs (250 μM 5^′-DFUR, 100 nM gemcitabine for MCF7 and 50 μM gemcitabine for HT29), non-transfected (NT), negative control siRNA (NC) or AQP3-siRNA (siA) transfected MCF7 (a) or HT29 (b) cells were stained with propidium iodide, and cell cycle was analyzed with a flow cytometer. Results are presented as means of four to six independent experiments. Statistical significance was assessed with the Student’s t test (* p < 0.05; ** p < 0.01).