Figure 2
Infectivity, induction of syncytia, and CPE of MV-Edm in human HB cells. (a) Serial analysis to determine the CPE of recombinant MV-Edm was performed every 24 hours on the human HB cell lines Hep2G and HUH6 and normal liver cell line L-02. Seventy-two hours after infection at MOIs of 0.1 and 1, the cells were stained with crystal violet representing viable, attached cells. (b) The time course of cell viability of the human HB cell lines Hep2G and HUH6 and normal liver cell line L-02 (n = 8) after infection with recombinant MV-Edm at MOIs of 0.1 and 1 was analyzed using a Cell Titer 96 Aqueous nonradioactive cell proliferation assay kit. ***GroupMOI=1.0 versus Group MOI=0, P < 0.05; ** GroupMOI=0.1 versus Group MOI=0, P < 0.05. * GroupMOI=0.01 versus Group MOI=0, P < 0.05.