Copy Number Amplification of the PIK3CA Gene Is Associated with Poor Prognosis in Non-lymph node metastatic Head and Neck Squamous Cell Carcinoma

Table 1 Primer sequences and annealing temperatures for direct sequencing

Gene	Exon	Primer sequence	Tm,
PIK3CA	9	F,5’-TTGCTTTTTCTGTAAATCATCTGTG-3’	51
		R,5’-CCACAAATATCAATTTACAACCATTG-3’
	20	F,5’-GGTATTAACATCATTTGCTCCAA-3’	52
		R,5’-CCTATGCAATCGGTCTTTGC-3’
KRAS	1	F,5’-CATTACGATACACGTCTGCAGTCAACTGG-3’	52
		R,5’-GTGAACATCATGGACCCTGACATACTCC-3’
BRAF	15	F,5’-TCATAATGCTTGCTCTGATAGGA-3’	52
		R,5-GGCCAAAATTTAATCAGTGGA-3’

F, forward primer R, reverse primer Tm, annealing temparature.
We used DNA sequencing to detect mutations in the clinical HNSCC specimens. KRAS (exons 1 and 2), BRAF (exon 15) and PIK3CA (exons 9 and 20) were analyzed for mutations.

ISSN: 1471-2407