Figure 5

ATO inhibits thymidylate synthase expression in a dose dependent fashion. HepG2 cells were treated for 24 hrs with vehicle, 0.5 μM, 5 μM or 25 μM ATO. Thymidylate synthase mRNA expression was assessed by qRT-PCR and normalized to actin levels. Each sample was run in triplicate. A representative example of four independent experiments is shown. Bar graph demonstrates fold change relative to vehicle control +/− SEM.