Figure 5

Intracellular localization of doxorubicin and doxorubicinol in MCF-7 _CC12 and MCF-7 _DOX2-12 cells upon treatment with or without 5β-cholanic acid. Cells were treated for 24 h with doxorubicin or doxorubicinol (red) in the presence or absence of the AKR1C2, AKR1C3, and AKR1B10 inhibitor 5β-cholanic acid. After treatment for 24 h, the nuclear dye DRAQ5 (blue) was added to the media as a counterstain for 15 minutes, and then coverslips were mounted and imaged by confocal fluorescence microscopy.