Figure 8From: Reovirus exerts potent oncolytic effects in head and neck cancer cell lines that are independent of signalling in the EGFR pathway Inhibition of PKR does not augment reovirus cytotoxicity in HN cells. A. SIHN 11B, B. PJ41, C. HN3, D. HN5, were incubated with the PKR inhibitor 2-aminopurine (2AP) for 1hr prior to infection with reovirus (MOI 50) using viral stocks at 7.8×108 TCID50/ml. Cell survival was assessed by MTT assay at 96 hours post-infection. Data are representative of 3 independent experiments and error bars are SEMs. E. L929, HN3, HN5, PJ41 and SIHN11b were plated at 1×106/10 cm dish and treated the following day with 2AP (1 mM). After 1 hour incubation with 2AP, reovirus was added at an MOI 50. Cells were incubated for a further 4 hours and then harvested for western analysis with p-EIF2α and γ-tubulin for loading controls.Back to article page