Figure 3

TIC protein marker expression in M- and S-type samples. A. Representative blot showing CD44 expression in M- and S-type samples. Based on all samples, CD44 was associated with M-type (p<0.001; Table 2). Numbers refer to patients. B. The SKOV-3 cell line was used for immunoprecipitation with antibodies to Oct-4 and to SUMO, respectively. Shown here is a representative western blot of three immunoprecipitates made with antibodies to Oct-4, SUMO and mouse IgG, respectively, and which were then probed using anti-Oct-4A. Mouse IgG served as negative control. SUMO-Oct-4A could be detected when probed with anti-SUMO (not shown). The results were confirmed using two other cell lines (not shown). Arrow: native ~40 kDa Oct-4A just above the 25 kDa IgG light chain. IP: immunoprecipitation; WB: western blot probe; m-IgG: mouse IgG.C. Oct-4A and SUMO-Oct-4A in M-type samples and paired samples of M- and S-type. D. Box plots comparing the distribution of relative expression of Oct-4A, SUMO-Oct-4A and Nanog, respectively, in all M (n=18) and S (n=9) samples. Asterisks denote statistically significant differences (Mann–Whitney U test).