Figure 1

EGFR-specific PIT in vitro. (A) MDA-MB-468luc cells were treated with Pan-IR700 and observed with microscopy (Before and after Pan-IR700). Necrotic cell death was observed upon excitation with NIR light (After). (B) Both EGFR positive (MDA-MB-468luc) and negative (Balb/3T3) cells were co-cultured and treated with Pan-IR700 (Before and after Pan-IR700). EGFR-specific necrotic cell death was observed (After). (C) NIR light irradiation results in dose dependent cell death as determined by the cytotoxicity assay. (n = 3, *** P<0.001 vs. non treatment control, Student’s t test).