Figure 2

Snail overexpression in 22Rv1 cells leads to decreased maspin expression, and increased migratory and invasive potential. 22Rv1 prostate cancer cells were stably transfected with Snail cDNA or empty vector control (Neo) using lipofectamine 2000. (A) A representative Snail clone (Snail-high) that expressed the highest levels of Snail, as assayed by PCR and Western blot analysis, also expressed the least amount of maspin, when compared to Neo control and the low-expressing Snail clone (Snail-low). (B) Snail overexpression in 22Rv1 cells was accompanied by increased migration on collagen and (C) increased invasion on matrigel in the Snail-high clone. Results are representative of three independent experiments. Data represent mean ± SD (* p < 0.05, **p < 0.01).