Figure 1

Protein expression of Stat1, P-Stat1 and FLAG in transfected cells. ( A) U3A cells were transiently transfected with the pcIneo vector, the wtStat1pcIneo vector and the Stat1C vector as indicated. The cells were left untreated or were treated with IFN-γ (1000 U/mL) for 24 hours. The cells were lysed and Western blot analysis was performed using the indicated antibodies. ( B) and ( C) Stably transfected U-266-1970 cells were left untreated or were treated with IFN-γ (1000 U/mL) for the indicated times. Protein lysates were prepared and Western blot analysis was performed using the indicated antibodies.