Figure 4

Silencing cdx-2 expression restores Ephrin-A1 mediated inhibition of Claudin-2 expression in NSCLC cells. Plate A: Relative mRNA expression of claudin-2 normalized to endogenous control. (pcDNA-EFNA1 is vector containing ephrin-A1 construct). Plate B: Claudin-2 expression was analyzed by Western blot, data represents mean ± SEM of three independent experiments. *p < 0.001 compared to control, # p < 0.001 compared to Empty vector, $p < 0.001 compared to cdx2-siRNA alone. **p < 0.001 compared to control. Plate C: Immunofluorescence analysis of claudin-2 expression in NSCLC. Blue colour represents the nuclear stain DAPI (4, 6-diamino-2-phenylindole), Red colour represents Rhodamin phalloidin for F-actin filaments, and green colour represents Alexa Flour-488 labelled for claudin-2 expression. The data presented is a single representative of three similar observations. Scale Bar = 40 μm.