Figure 3From: ZIC1 modulates cell-cycle distributions and cell migration through regulation of sonic hedgehog, PI3K and MAPK signaling pathways in gastric cancer ZIC1 inhibits the expression of Sonic hedgehog (Shh). (A) The expression of Shh protein was analyzed by Western blot in BGC823 and SGC7901 cells stably transfected with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector. (B) The expression level of Shh mRNA was determined by real-time quantitative RT-PCR analysis. The relative expression level was expressed as fold change (mean ± SEM) compared with pCDNA3.1 empty vector normalized to 1.Back to article page