Figure 2

Stabilisation of target genes in HT29, BRCA1 and BRCA2 positive control cell lines after GINI with caffeine (7.5mM) treatment. Three experimental replicates of the GINI method were performed on HT29 cells, BRCA1 c.2681_2682delAA and BRCA2 c.539_541insAcontrol LCLs. qRT-PCR results indicate that each experimental replicate shows at least a 1.5-fold stabilisation of all three target genes after treatment with 7.5mM caffeine. Each sample has been normalised to the housekeeping gene, GAPDH, and calibrated to its untreated equivalent. Error bars represent standard error of the mean of four technical replicates of each PCR reaction.