Figure 2
From: Microtubule S-glutathionylation as a potential approach for antimitotic agents
2-AAPA induces protein S -glutationylation in UACC-62 cells. UACC-62 cells were treated with 50 μM 2-AAPA for different time periods and protein S-glutathionylation was determined by the HPLC method described by Chen et al. [16]. The results were presented as the mean ± SD of triplet determination of one set of samples. No protein S-glutathionylation was detected in untreated cells. Control 1 and control 2 were conducted at 5 min and 4 h respectively. No S-glutathionylation was detected in both control 1 and control 2.